Protein isolation Lab Report Example | Topics and Well Written Essays - 500 words

Protein isolation - Lab Report ExampleThe aim of the experiment is to isolate, purify and identify Rubsico in spinach leaves using Ammonium sulphate, spectrophotometry, and electrophoresis and ion exchange techniques.Spinach leaves were described, homogenized and rubisco isolated using 2 grades of ammonium sulphate concentrations (37 % and 50%). Filt sites and supernatants were tested using appropriate techniques to ascertain successful isolation. (Robinson, Streusand, Chatfield and Portis, 1988) The isolate was hence purified using DEAE Cellulose fast flow ion exchange chromatography and bound proteins eluted using different shades of salt concentration. Purified proteins were give on a SDS-gel electrophoresis to identify the isolate (Robinson, Streusand, Chatfield and Portis , 1988).Ammonium sulphate (salt) precipitates proteins by altering total heat bond interaction between protein and wet molecules. Ammonium sulphate a high affinity for water molecules displaces protein mo lecules (lowers the solubility) thereby causing precipitation. Different protein molecules precipitate at different concentrations of ammonium sulphate and at the rate at which it is added. The meat of salt concentration on the isolation of rubisco is shown on figure 1. The absence of the rubisco slew on sample p2 (protein isolated at 50 % salt concentration) shows a significant amount of the protein was isolated at 37% concentrations of ammonium sulphate. A protein molecular weight ladder shown in figure 2 was used to establish a calibration curve that used to identify the protein isolate.Figure 1 The effect of salt concentration on the isolation of proteins the top arrows represent the samples ( from left to right MW ladder, leaf repeat filtrate, Supernatant of counterbalance precipitate (S37%), Pellet of first precipitate (S37%) at low salt, Pellet of first precipitate (S37%) at medium salt, Pellet of

Research some initiatives that reduce the devasting impact that Essay

Research some initiatives that reduce the devasting impact that hospitality operations deport on our environment - Essay ExamplePolitical rationality is based on the ability to solve problems and land at effective, collective decisions. Technical rationality is dependent on the value of efficiency. These modern environmental guidance methods require a rationality of both means and ends. They specify the means by which given ends coffin nail be achieved. Those ends are environmental goals such as less pollution and the conservation of water.There is a close relationship between hotels and the environment. Various features of the physical environment are an attraction for tourists. The environment covers only of peoples natural and cultural surroundings. The natural environment is what exists in nature such as the land and its blot and topography. The built environment consists of man-made physical features consisting of infrastructure development. Tourist facilities and infrastr ucture constitute one facial gesture of the built environment. The ensuing tourism development and tourist use of an demesne generate environmental impacts.Hotels have signified their commitment to ensure a healthy and clean environment. There are three internationalist hotels which have implemented very successful environmental initiatives. The Shangri-la Hotels and Resorts Hong Kong has initiatives on environmental counseling. In the area of environmental sustainability, the hotels focus is primarily in five areas climate change ozone depletion water use management waste disposal management and indoor air quality. promised land is ramping up nil conservation initiatives so that it will reduce 2006 company-wide energy consumption figures by 12 percent by the end of 2008. Currently, the group practices a wide array of environmentally friendly measures such as fitting all guestrooms with water-saving devices in taps and showers as well as using energy-saving lamps in more tha n half of the groups guestrooms. Shangri-La was an pains leader in initiating environmental procedures even before its hotels began to receive ISO14001 certification, the international Environmental Management frame Standard. The group has an extensive list of internally developed environmental best practices that all hotels have implemented, with inspections to admonisher for compliance. The inspection results affect each hotels annual performance and development review scores. In addressing impacts on climate change, the group is working on reducing carbon dioxide emissions per room night by 6.7 kg., as compared to 2006 figures. Shangri-La has three broad methods for reducing emissions of greenhouse gases by existing systems and processes the use of new technologies and alternative energy sources and by building more efficient buildings. In new hotels, Shangri-La seeks to save energy and resources through a variety of techniques involving rainwater harvesting, hot water produc tion using solar panels or set off pumps, and improvements in building envelope design to reduce heat loss or heat evolve as well as air filtration. (Shangri-la Company website). Another international hotel with a sterling character on environmental

The last supper by Leonardo Da Vinci Essay Example | Topics and Well Written Essays - 1500 words

The last supper by Leonardo Da Vinci - Essay ExampleThe ab show up noniceable aspect of Florence was that it was a self governed and approximately importantly an independent urban center. ( conversion)The city grew with leaps and bounds because of its tough economic background to match this, the city also boasted of a strong political philosophy, these factors contributed in the ample mastery of Florence during the period of the Renaissance. The economy of Florence was centered on the manufacturing of goods or trade of cloth so needs meant that the most powerful nation in the city had to be the people who represented the textile workers. Workers in Florence were experts when it came to transforming wool into cloth of an excellent quality they wee well acquainted with the ways to do the same. The outgrowth was a quite complicated one which involved dying of wool, cleaning the wool and a waiter of new(prenominal) processes.The Palazzo Vecchio was constructed in the year 129 9. This was also the home of the Florentine guilds. This portrays the Florentine culture that was the home of umpteen a(prenominal) Italians. This was a place where influential people would gather and decide complex issues like voting and other important things. The guild members were all from good backgrounds, the background of the people was the most important factor that refractory whether they were good enough to become guild members or not. Some guild members were lawyers, sculptors while other members include solicitors, masons and builders. Florence was a very different city it was unlike Venice or for that matter any other city during the term of Renaissance. ocean trade did not flourish in Florence as much as it did in Venice because the people of Florence had many other interests which fetched them much more than Sea trade, this was primarily the reason why Sea trade never flourished in Florence. Banking was one of the most common professions during the time of Renaiss ance many families tasted success being bankers in Florence. The gold coin Manufactured in Florence was a trademark of purity and was recognized the world over by people. The buildings and the sculpture are very famous and looked upon by many people all across the globe, some of the buildings built at that time still stand, his goes to show the pain and the efforts which were out in by the people at that time. Renaissance was a revolutionary period which saw rattling(a) changes take place in Europe. Renaissance is a French word which means rebirth many a thing changed during the period of renaissance for instance the Political changes in Europe, social changes which took place during that time etc. This paper will throw light upon Grnewalds Isenheim Altarpiece. Grnewald was a very prolific artist of the Renaissance period he was mainly involved in making religious paintings. This paper will offer an insight on his masterpiece which is known as the Altarpiece which is now located in France. In addition to this the paper will also throw light upon two other masterpieces. (Grnewalds Isenheim Altarpiece)Mathis was one of the most famous painters of the Renaissance period. He was not well known until the late 19th century which brought him to the limelight. The details about this great artist are still obscure, for the reputation that he had back then, people reckon a lot of information about him but the fact is that even his real name is not known by the people. The name

Photography Research Paper Example | Topics and Well Written Essays - 4000 words

Photography - seek Paper ExamplePart 3 Digital photography has changed the way we see the world nigh us. It has exposed up the world of photography to anyone with the ability to peer through a viewfinder and drudge a button. It has made it possible for us to easily pull shows into our computers and change the captured reality. For this reason, it has become a dread that digital photography has changed the face of photography forever. We can no longer look at an figure and suck in that it is depicting what in truth happened at the time the photograph was taken. Many people remain jealous virtually digital photography because of the ease with which photos can be cookd, but this aspect of photography hasnt really changed since its soonest days. Part 3 Digital photography has changed the way we see the world around us. It has opened up the world of photography to anyone with the ability to peer through a viewfinder and push a button. It has made it possible for us to easily pull images into our computers and change the captured reality. For this reason, it has become a concern that digital photography has changed the face of photography forever. We can no longer look at an image and assume that it is depicting what really happened at the time the photograph was taken. Many people remain suspicious about digital photography because of the ease with which photos can be manipulated, but this aspect of photography hasnt really changed since its earliest days. By looking at the history of photography and how it has been affected by digital technology, I platform to show that manipulation of photos is something that has been a part of photography for as long as cameras existed. To do this, I will show how the function of the digital camera is not really that different from the function of the handed-down take in camera and then show how each method is easily manipulated to create an impression of reality. The hardly true difference is our realization tha t this is possible. Digital Technology Has Not Changed Photography Student fig play name Instructor name Date Student name Instructor name Course name Date Digital Technology Has Not Changed Photography The world as we see it today is broadly speaking shaped by what is captured in the form of digital photography. Film cameras are al almost non-existent as to a greater extent and more people have found the ease and cost-convenience of digital to far outweigh the hassles of film for most applications. Because of its widespread use, we are all now much more aware of just how easy it is to manipulate photographs to change what we think we see. While most of us embrace the ease of digital photography for ourselves, on that point is a group of individuals who are outraged at the idea that news lensmans are also equal to use digital technologies in reporting the news. These people feel that the only way we can drive an accurate representation of whats happening in other parts of the world is through the use of film cameras, which produce images that are not as easy to manipulate. My role in the following research is to study how film cameras and digital cameras operate to see if there are any differences in the actual capturing of an image and then to compare how images are processed, again with the purpose of comparing techniques and the level to which they can be manipulated. In conducting this research, I bring my own experience as a film and digital amateur photographer as well as someone interested in how the media works to shape our understanding of the world. I have studied how images are used in print ads, video

Illegal Software Use Essay Example | Topics and Well Written Essays - 1250 words

unratified Softwargon Use - Essay ExampleThe 2011BSA Global Software Piracy Study reveals that the Business decision-makers who make they frequently pirate bundle are more than twice as likely as other(a) computer users to say they buy software package for one computer but then install it on additional machines in their offices,. The latest study reveals that a number of security breaches come by know hackers. On this numerous reports including patches and software updates are presented. Moreover, a questioned is asked for the presence of relationship between unlicensed software and the perseverance of software is maintained or not? In this regard the CEO Robert Holleyman, president BSA says, It piece of ass be a security issue. Furthermore, he states that there is a major cultural problem that leads towards use pirated software and other security breaches. However, in any organization the security lapse can to a fault happen due to mismanagement of its software and licensing. The unlicensed and not properly documented software are more prone to provided insufficient support to the organization. According to the Holleyman, the BSA report is founded over the IDC merchandise data. The term amber standard is stated by the Hollyman that supports the sales of PC and software in worldwide markets (GNC Articles. 2012). This information is cool and published by Ipsos Public Affairs. The survey is held between 14,700 individuals that belong to 33 countries therefore representing more or less 80 share of the worldwide software market (GNC Articles. 2012). The survey results came up with some astonishing facts. Around cardinal two percent of individuals had installed he pirated version of software that cost around $63.4 billion (GNC Articles. 2012). However, this cost cannot be claimed as the net loss due to the unknown budget of legal or illegal installed software as mentioned by BSA in the software industry. In the United States, the rate of pirated software is about nineteen percentages because it is considered as the most law-abiding country percentage-wise. In fact, for the big illegal software shares in the U.S. market the estimated net worth is approximately around $9.8 billion value. In the second place is China (GNC Articles. 2012). It is verbalise that the country pirates an approximated 77 percent of its software. In addition, this software is of worth about $8.9 billion that stands China in the second place of illegal software after United States. The computer users in China throw out on typical just $8.89 for the legal software and in the United States this figure is around $127 for every computer. According to the BSA these cost figures for the software organizations are representing risk to U.S. economic well-being. This is due to the unwarranted militant advantage that is given to the industries thus encouraging them to use the pirated software. Likewise, even at the rate of 19 percent software piracy in the United Sta tes poses a major threat to the Information and Communication engineering in the United States (GNC Articles. 2012). Firstly, applications or software coming from a non-genuine source can be septic with viruses, malicious codes, Trojan horse, root kits etc. this imposes a direct threat or risks. For instance, if an operation arrangement has been acquired from a non-genuine source, viruses can be installed with the operating system. Likewise, one cannot trust software provided by the vendor and it should be tested before implementing in a live or operational environment of a coast or financial institution. These fixes can be later on patched by security updates. In case of pirated software, update options are turned off due to the fear of Operating system organization tools from the manufacturers. Once

Literature- Discussing Questions 1 Essay Example | Topics and Well Written Essays - 1000 words

Literature- Discussing Questions 1 - Essay ExampleShe appears plain and hard-working in this first scene.Henry and enzyme-linked-immunosorbent serologic assay seem to be a settled couple. He cares how she feels and she cares for him also, unless there seems to be no dear there. Instead they are good friends, but non intimate in the sharing sense of the word. She does not want him to know about the mums. He seems to value her skills and strength, rather than her femininity, and he says she looks nice, not beautiful.The salesman takes and take in order to establish rapport, and to get some work from her, plus a flowerpot he can sell. She is taken in, believes his story and thinks he has a genuine interest in her flowers. It is her specialty and she loves sprightliness like she is sharing it. (Ten inch mums are huge.)The first paragraph describe Elisas popular opinion about her life closed in, grey and damp, waiting for something to change. She is isolated and her life is quite l imited and simple, as opportunities for women of that time were very limited.The valley is ready to sleep through the coming wionter in anticipation of spring, but Elisa has passed her chance at spring and new blooms. She lives that part of her life through her flowers.5 How qualification Elisas preparations for going out for dinner be likened to her care for the chrysanthemums? Explain Elisas response after seeing her seedling chrysanthemums become been tossed out onto the road.Elisas preparations for going out to dinner are meticulous and thorough. She does not only care for her own body, but for her husbands clothing too. When she is done bathing she surveys the results and then dresses. She is as careful with her flowers, as we see in the first scene and she tells us with her directions to the salesman for taking care of them. She jam-packed the seedlings with great care for him to take to the fictional client. She realizes that he has manipulated her and just wanted the money and the flower pot, which she notes that he

Reflections on Contemporary Leadership Issues and Strategies Essay

Reflections on Contemporary Leadership Issues and Strategies - Essay typeThe application of various concepts in the practical world allows one to understand the functionality and dynamic nature that leaders requires, in order for this subtle art to be effective. The class boasts of a plethora of different nationalities and backgrounds that bemuse made me appreciate key ideologies like teamwork, motivation and appreciating diversity amongst many other.Leadership is not notwithstanding about managing people and increasing productivity to generate higher profit levels. It is an amalgamation of various individualised, kindly and strategic skills. True skills promoted by leaders are developed over a period of time, and are evolved through regular use in all aspects of the daily routine of an individual. This by no federal agency implies that the same skill set will apply and work in all situations. While authorized individuals possess inherent leadership qualities and abilities, o thers will need to perfect their skills through learning and practicing. One sign which must be remembered by all is that true leadership values can never be taught they must be groomed through use.The feedback provided by my fellow colleagues was an enlightening experience that allowed for an understanding from a different perspective. Conversations and discussions allowed everyone to contribute to the matter in an open environment, with each individual able to discuss their views and experiences of leadership. This provided a more comprehensive outlook of the subject, with examples of various industries and sectors like information technology (IT), health care, hospitality, human resources (HR) and facial expression from the public and private aspects, with global and local perspectives.Everyone has a different way of relating to/with leadership. I believe that my personal reflection varies from most of the generic scenarios. Most of the course text assigned to us provided detai led discussions on leadership from a business outlook. While these harnessed ideas and

International business enviroment Essay Example | Topics and Well Written Essays - 2750 words

International business enviroment - Essay ExampleAccording to the literature on world(prenominal) business, FDI level is dependent on the effects of location of the business environment at heart a country. referable to uneven distribution of resources in a certain country, the firms will have contrastive advantages in different countries (Hufbauer 2013, p. 1).Studies carried out on determinants of inflow FDI reflects a positive correlation between the economic performance and FDI. muddle factors explain the differences in foreign penetration between countries. According to Pentecost and Miner (1996), competitive advantage deep down the host economies, levels of protection and the size of the inform market affects the distribution of FDI positively. Studies also show that FDI is significantly stirred by the policy measures directed at FDI (Lee 2001, p. 100).The current paper uses a range of relevant theories and selective information as illustrated in Tables 1 and 2 to critica lly evaluate variation over time between and within countries in their inflow and outflow of FDI activity. In this case, FDI flows as a percentage of the GDP. These indicators are a representation of the provision of the external financing resources in terms of direct investments within a reporting economy derived from foreign investors and external economies as derived by the internal investors. The negative FDI values of net inflows within a certain country is a reflection of the disinvestment value from foreign investors being higher than bang-up value of investments within the reporting country. The negative value for the net outflows indicate that value derived from the direct investments from domestic investors to the external economies is less than the repatriated value of the direct investments from the external economies (Rutten and Boekema 2007, p. 39).Most countries use FDI for evaluation of their economic training processes. The contribution of FDI to national output and

Impact of Globalization on General Motors Essay

Impact of Globalization on General Motors - Essay ExampleThese include simplifying the process where possible, conclusion cheaper but high quality material to help with the manufacturing process (Reader 2006, p.7). Additionally the confederacy believes that innovative engine room will help it attain better business hence increasing output but the read/write head is how globalisation in the automotive industry affects GM. Globalisation is a double-edged sword that cuts both(prenominal) ship canal in this case it has both the positive and the negative impact on any industry. However, the degree of managing these personal effects determines the success or failure of a company in a particular industry. When examining the effects of globalisation in a company for instance GM, we have to look at all the department, branches, plants and any subsidiaries of the company. The priming coat for this is that globalisation is a multifaceted phenomenon. For instance, we have to look at how globalisation has affected GM employees, the production process, the marketing aspect, the information technology aspect and the management aspect (Alias 2008, p.10). The complexity of the automotive industry demands that the company should effectively deal with the impact of globalisation because if the competitors ar able to deal with it more effectively than the GM then this can be catastrophic or detrimental to both the company image and the market. The public is moving towards becoming a global village hence having a very fast station of globalisation. As a result, companies should strategise and gear up for this cause to avoid eradication referable to drastic changes. However, in dealing with the effects of globalisation on the GM it is important to separate all the sections and critically examining them singly ratherthat having a... The paper tells that increased globalisation has led to increased competition in the automotive industry with adaptation of new entrants w ho have better ways of manufacturing and production of vehicles. However, one of the biggest competitors to GM is the Japanese company Toyota manufacturing undestroyable and affordable vehicles. Additionally, due to the persistent hard economic times more and more people are opting to buy fuel-efficient vehicle and vehicles whose spare parts are easily available. The Toyota Company has managed to tap into this market both locally and globally hence giving GM very stiff competition. Additionally, due to globalisation vehicles are becoming cheaper hence reducing the profit margin of GM. Because of this, it forces the company to seek ways of cutting crush costs in regularise to increase the profit margin because they cannot increase the prices due to the demand and offer forces of the market. Globalisation has in addition made information readily available to consumers in the market. Additionally, the introduction of ecommerce solution has also cut down on the cost of business tra nsactions. Customers are now able to purchase vehicles from some other counter through the internet and go through all the relevant processes that will enable him or her get the vehicle at his or her disposal. GM has to deal with these globalisation effects in order to stay afloat in business. The distance between countries is closing up very fast due to the development of efficient ways of transportation and business transactions are becoming easier due to the proficient development.